Real-time PCR assay for trichomonosis on pooled direct preputial samples

Authors

  • A. Garcia Guerra Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada, S7N 5B4
  • J. E. Hill Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada, S7N 5B4
  • J. Campbell Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada, S7N 5B4
  • C. Waldner Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada, S7N 5B4
  • S. Hendrick Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada, S7N 5B4

DOI:

https://doi.org/10.21423/aabppro20123925

Keywords:

Tritrichomonas foetus, reproductive management, PCR methods, Areal-time PCR, pooled samples, preputial samples

Abstract

Tritrichomonas foetus is recognized as important cause of reproductive loss in beef herds. Strategies for the control of trichomonosis (trich) in beef herds are based on identifying and eliminating infected bulls. The sensitivity of conventional PCR methods is sufficient for use of detecting trich in pooled preputial samples from up to five bulls. Areal-time PCR (RT-PCR) assay for trich detection has shown improved sensitivity, compared with that of the conventional PCR assay. Previous research from our group demonstrated that pools of samples from up to 25 bulls, enriched by culture, could be tested for trich with the RT-PCR assay without a significant decrease in sensitivity. The objective of the present study was to evaluate the sensitivity of a commercially available RT-PCR assay for the detection of trich in pooled samples of direct preputial samples from five or 10 bulls.

Cover image of the 45th Conference Proceedings: Three black-and-white cows lounge in a spring field. Yellow background.

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Published

2012-09-20

Issue

2012

Section

Research Summaries 3

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