Detection of Bovine Respiratory Syncytial Virus Using Commercial Human Respiratory Syncytial Virus Rapid Immunomigration Assays

Authors

  • N. Barbu Department of Large Animal Clinical Sciences, Michigan State University, East Lansing, MI 48824
  • C. Passmore Department of Pathobiolgy and Diagnostic Investigation, Michigan State University, East Lansing, MI 48824
  • R. Urban University of Life Sciences in Lublin, Poland
  • R. Maes Department of Pathobiolgy and Diagnostic Investigation, Michigan State University, East Lansing, MI 48824
  • D. Grooms Department of Large Animal Clinical Sciences, Michigan State University, East Lansing, MI 48824

DOI:

https://doi.org/10.21423/aabppro20104140

Keywords:

Bovine respiratory syncytial virus, BRSV, pneumonia, immunofluorence, ELISA, RT-PCR, patient-side assay, diagnosis, control plans, immunomigration, human respiratory syncytial virus

Abstract

Bovine respiratory syncytial virus (BRSV) causes a severe interstitial pneumonia primarily in calves from birth to 12 months of age, and is a significant component of the bovine respiratory disease complex. Current detection of BRSV is performed by laboratory-based methods including direct and indirect immunofluorence, real time-polymerase chain reaction (RT-PCR), or enzyme-linked immunosorbent assay (ELISA). The development of a rapid patient-side BRSV detection assay would facilitate timely disease diagnosis and the institution of appropriate therapeutic and control plans. Rapid immunomigration assays are readily available for the detection of human respiratory syncytial virus (HRSV). BRSV and HRSV share significant genetic and antigenic homology. Taking advantage of this homology, this study investigated the use of commercially available HRSV immune migration assays to detect BRSV.

Cover image of the 43rd Conference Proceedings: Two short-horned cattle grazing in a field, surrounded by a flock of egrets. Yellow background.

Downloads

Published

2010-08-19

Issue

2010

Section

Research Summaries 3