Genetic and Antigenic Characterization of Bovine Viral Diarrhea Field Isolates

Authors

  • B. M. Hause Newport Laboratories, Worthington, MN 56187
  • R. F. Bey Newport Laboratories, Worthington, MN 56187
  • D. L. Stine Newport Laboratories, Worthington, MN 56187
  • R. R. Simonson Newport Laboratories, Worthington, MN 56187

DOI:

https://doi.org/10.21423/aabppro20104143

Keywords:

Bovine viral diarrhea virus, BVDV, vaccines, genotype, field isolates, E2 gene, antigenic methods

Abstract

Bovine viral diarrhea virus (BVDV) is commonly found in cattle and causes significant economic losses. Despite long term and widespread use of commercial modified live vaccines, BVD remains problematic. BVDV are classified into two genotypes, BVDV-1 and BVDV-2, and are further subdivided within the genotype by sequence analysis. Most genetic analysis is performed on the 5'-untranslated region of the genome as it is relatively conserved, however, this region does not code for an antigen. The objective of this study was to characterize recent BVDV field isolates using genetic analysis of the E2 gene in combination with antigenic methods using antisera generated against multiple isolates in a high throughput serum neutralization assay.

Cover image of the 43rd Conference Proceedings: Two short-horned cattle grazing in a field, surrounded by a flock of egrets. Yellow background.

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Published

2010-08-19

Issue

2010

Section

Research Summaries 3