The Effect of Sample Handing on Test Results for Antigen Capturing ELISA for BVDV

Authors

  • M. C. Reed VDPAM, Iowa State University, Ames, IA
  • A. M. O'Connor VDPAM, Iowa State University, Ames, IA
  • K. J. Yoon VDPAM, Iowa State University, Ames, IA
  • V. Cooper VDPAM, Iowa State University, Ames, IA

DOI:

https://doi.org/10.21423/aabppro20074589

Keywords:

diagnostic sample, handling practice, ELISA, bovine viral diarrhea virus, BVDV, ear notcher, virus accumulation

Abstract

Certain handling practices of diagnostic samples may affect the sensitivity and specificity of the antigen capturing ELISA (ACE) for bovine viral diarrhea virus (BVDV). Leaving samples at room temperature for an extended period of time may have a negative impact on test sensitivity, and failure to wash the ear notcher between subsequent skin sampling may lead to virus accumulation and may decrease test specificity. We tested these hypotheses in two studies by collecting known positive and negative samples and giving groups of samples different treatments.

Cover image of the 40th Conference Proceedings: A single longhorn standing next to a barbed-wire fence on a cloudy day. Yellow background.

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Published

2007-09-20

Issue

2007

Section

Research Summaries 2

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