Sample Handling Substantially Affects Johne's ELISA

Authors

  • C. A. Alinovi Department of Veterinary Pathobiology, Purdue University, West Lafayette, IN 47907
  • M. P. Ward Veterinary Integrative Biosciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843
  • E. A. Raizman Department of Veterinary Pathobiology, Purdue University, West Lafayette, IN 47907
  • T. L. Lin Department of Veterinary Pathobiology, Purdue University, West Lafayette, IN 47907
  • C. C. Wu Department of Veterinary Pathobiology, Purdue University, West Lafayette, IN 47907

DOI:

https://doi.org/10.21423/aabppro20064764

Keywords:

enzyme-linked immunosorbent assay, Mycobacterium avium subspecies paratuberculosis, Johne's disease, sample handling

Abstract

The Johne's enzyme-linked immunosorbent assay (ELISA) is designed for rapid detection of antibody to Mycobacterium avium subspecies paratuberculosis (MAP), the causative agent of Johne's disease in ruminants. However, there can be significant variation in ELISA values between wells, test kits and laboratories. The objective of the present study was to determine if sample handling could significantly affect ELISA values to MAP.

Cover image of the 39th Conference Proceedings: Three calves lying down in a pasture next to a white fence. Behind them, a rainbow spans over the distant hills. Yellow background.

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Published

2006-09-21

Issue

2006

Section

Research Summaries 4

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