In vitro Fertilization of Cumulus-Free Bovine Oocytes in Caffeine and Different Concentrations of Calf Serum

Authors

  • P. Tajik Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, P.O. Box 14155-6453, Tehran, Iran
  • K. Niwa Division of Animal Science and Technology, Faculty of Agriculture, Okayama University, Okayama 700, Japan

DOI:

https://doi.org/10.21423/aabppro20015274

Keywords:

in vitro fertilization, fetal bovine serum, bovine serum albumin, calf serum, heparin, bovine oocytes, caffeine

Abstract

Bovine in vitro fertilization (IVF) has been of great importance and the subject of different studies in animal lab oratories around the world. Different protein supplements have been used for IVF in these laboratories. In our previous study2 fetal bovine serum (FBS), bovine serum albumin (BSA) and calf serum (CS) had been added to fertilization medium containing caffeine and heparin, and different results were observed. One of the most interesting points was that, in 20% CS, limited penetration rates were observed in media containing caffeine and heparin. In the present study, the same concentrations of CS were added to the fertilization medium, with or without caffeine, to study whether exclusion of heparin can support IVF of cumulus-free bovine oocytes in high concentrations of CS.

Cover image of the 34th Conference Proceedings: Four men on horseback lead a single cow through a pen. Yellow background.

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Published

2001-09-13

Issue

2001

Section

Posters

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