Comparison of Three Reverse Transcription-Polymerase Chain Reaction Assays for the Detection and Genotyping of Bovine Viral Diarrhea Virus Isolates

Authors

  • Byung Chul Ahn Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506
  • Paul H. Walz Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506
  • Sanjay Kapil Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506

DOI:

https://doi.org/10.21423/aabppro20015280

Keywords:

Bovine viral diarrhea virus, BVDV, transcription-polymerase chain reaction

Abstract

Bovine viral diarrhea virus (BVDV) is an important infectious disease in cattle. Isolates of BVDV are classified into type I or type II based upon the genomic sequences. Several reverse transcription-polymerase chain reaction (RT-PCR) techniques utilizing different genomic regions, such as 5'UTR, E0, and NS5B, have been developed for detecting and genotyping BVDV isolates. The purpose of this study was to compare the agreement among RT-PCR assays based upon these three different BVDV genomic regions in detecting and genotyping BVDV field isolates.

Cover image of the 34th Conference Proceedings: Four men on horseback lead a single cow through a pen. Yellow background.

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Published

2001-09-13

Issue

2001

Section

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