Comparison of Two Assay Techniques to Study In Vitro Phagocytosis of Bovine Granulocytes Using Flow Cytometry

Authors

  • D. Caudell VA-MD Regional College of Veterinary Medicine, Blacksburg, VA
  • S. McCourt VA-MD Regional College of Veterinary Medicine, Blacksburg, VA
  • J. Kalnitsky VA-MD Regional College of Veterinary Medicine, Blacksburg, VA
  • D. Ward VA-MD Regional College of Veterinary Medicine, Blacksburg, VA
  • N. Sriranganathan VA-MD Regional College of Veterinary Medicine, Blacksburg, VA
  • K. E. Saker VA-MD Regional College of Veterinary Medicine, Blacksburg, VA

DOI:

https://doi.org/10.21423/aabppro20005427

Keywords:

bovine pasteurellosis, pharmaceutical effects, in vitro assay, flow cytometry, cell response, phagocytic cells

Abstract

The response of phagocytic cells to Pasteurella haemolytica is difficult to study in situ. Available assays to study the function of these cell types often do not accurately reflect the physiology of the cell in response to bovine pasteurellosis. This study compared granulocyte phagocytosis of fluorescent beads and fluorescent bacteria using flow cytometry. Development of an in vitro assay would provide a model to study nutrient and pharmaceutical effects on the immune response to Pasteurella infections. It would also allow for future studies of the pathogenesis of not only bovine pasteurellosis but other diseases.

Cover image of the 33rd Conference Proceedings: Cattle and calves standing in a field of wildflowers, gazing curiously at the photographer. Yellow background.

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Published

2000-09-21

Issue

2000

Section

Posters

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