Evaluation of Nitric Oxide Production by Bovine Alveolar Macrophages

Authors

  • G. L. Mason Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO. 80523
  • P. N. Bochsler Department of Pathology, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37901

DOI:

https://doi.org/10.21423/aabppro20005428

Keywords:

nitric oxide synthase, microbicidal activity, Bovine alveolar macrophages, pneumonic lung, viral infection, macrophage permissiveness

Abstract

Expression of inducible nitric oxide synthase (iNOS) and production of nitric oxide (NO·) is a key defensive response of rodent macrophages against taxonomically diverse infectious agents in vitro and in vivo.1,4 Bovine alveolar macrophages (bAM) express iNOS in response to stimuli known to be present in pneumonic lung, but the role of NO· production in infectious pneumonia of cattle remains unknown.2,3,4 This work was designed to: i) evaluate the microbicidal activity of NO· against P. haemolytica Al, ii) determine if virus infection of bAM alters subsequent NO· production, and iii) determine if activation of bAM for NO· production alters macrophage permissiveness for subsequent viral infection.

Cover image of the 33rd Conference Proceedings: Cattle and calves standing in a field of wildflowers, gazing curiously at the photographer. Yellow background.

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Published

2000-09-21

Issue

2000

Section

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