Evaluation of the Test Characteristics of the HyMast® Bacteriological Test System

Authors

  • J. T. Jansen Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada
  • D. F. Kelton Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada
  • K. E. Leslie Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada
  • J. Ten Hag Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada
  • A. Bashiri Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada

DOI:

https://doi.org/10.21423/aabppro19995499

Keywords:

Mastitis, control programs, HyMast® test, diagnostic testing

Abstract

Mastitis continues to be a disease of major economic importance to the dairy industry, despite the wide use of mastitis control programs. Approaches to mastitis therapy may range from conservative to aggressive, depending on cow and management factors. Knowledge of the organism, prior to treatment, would appear to be of benefit in selecting the most appropriate course of therapy. The HyMast® test (Pharmacia & Upjohn, Kalamazoo, Michigan) is a selective media bacteriological test system for detection of gram-positive (Staphylococci, Streptococci) and gram-negative (coliform) organisms in milk. Although the primary use of this test has been for decision making in mastitis therapy, there is interest in identifying specific organisms from the test. The objectives of this study were to evaluate the sensitivity and specificity of the HyMast test read by producers on-farm and by readers in-clinic at 3 time periods, compared to standard milk bacteriology.

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Published

2020-03-23

Issue

Section

Research Summaries 1

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