Results of Virological Examinations of "Feedlot" Specimens During 1973 at TVMDL

Abstract

Effective virological diagnostic services have long been hampered by the time-consuming assay procedures. However, in the last few years great advances have been made and the time lag between request and answer have been shortened. This has been mostly due to the use of the FA techniques identifying a viral isolate. We no longer apply, at least on a regular basis, the FA test directly to tissue sections; even so, this would obviously be the fastest way in arriving at a diagnosis. The drawbacks with this method are nonspecific staining and low sensitivity. We inoculate all specimens for virological examination onto fetal125bovine cell cultures. These cell cultures are then stained with conjugate two to three and three to five days after inoculation for IBR and BVD, respectively.