Novel ELISA for detection of Neospora-specific antibodies in cattle
DOI:
https://doi.org/10.21423/aabppro19975847Keywords:
enzyme-linked immunosorbent assay, ELISA, Neospora species, Neospora caninum, bound antibodyAbstract
An enzyme-linked immunosorbent assay (ELISA) to detect antibodies to Neospora species in cattle was developed. Whole formalin-fixed Neospora caninum (NC-Liverpool) tachyzoites were used as antigen and a monoclonal antibody to bovine immunoglobulin light chain and an anti-mouse horseradish peroxidase conjugate were used to reveal bound antibody.
