Effect of Certain Antibiotics on the Bacterial Load and Fertility of Cattle and Buffalo Semen

Abstract

Semen samples were collected aseptically from 11 Native and 4 buffalo bulls. All animals were clinically healthy and free from brucellosis, tuberculosis and trichomoniasis. Bacteriological identifications, total viable bacterial count (T.V.B.C .) and antimicrobial sensitivity tests were done from the raw semen before evaluation. Six different antibiotics were added to equal portions of egg yolk buffer citrate solutions and another sample was kept free as control before semen extension. Only samples with initial individual motility more than 60% were subjected to cooling and preservation at 4 °C for further studies.