Rapid Detection of Bovine Viral Diarrhea Virus in Viremic Cattle by Flow-Cytometry

Authors

  • G. Wolf lnstitut für Med. Mikrobiologie, Infektions- und Seuchenmedizin, and ll. Med. Tierklinik, Tierärztliche Fakultät der Universitst, D-8000 München 22, Germany
  • G. Rademacher lnstitut für Med. Mikrobiologie, Infektions- und Seuchenmedizin, and ll. Med. Tierklinik, Tierärztliche Fakultät der Universitst, D-8000 München 22, Germany

DOI:

https://doi.org/10.21423/aabppro19926439

Keywords:

leukocyte analysis, viremic cattle, Flow cytometry, immunofluorescence, BVDV

Abstract

A fast and simple assay with high sensitivity to BVDV in blood samples independent of blood storage and clinical status of animals. is described. A pan-pesti virus reactive monoclonal antibody Cl6 (BVDV-p 80/125 specific) was applied for immunofluorescence (IF) in flow cytometry with leukocytes and for IF with infected bovine kidney cells as a reference method. From 304 blood samples of patients of the veterinary clinic 63 NCP- and 7 CP- BVDV strains were isolated. There is a high BVDVspecific IF in granulocytes and monocytes, whereas in lymphocytes the IF intensity per cell is weaker. The percentage of antigen positve cells varies from 1% to 87 % (mean 22%) in granulocytes/monocytes and 0% to 37% (mean 8%) in lymphocytes. There is a total correlation between conventional BVDV-diagnosis with tissue culture isolation and leukocyte analysis with flow cytometry. After blood freezing or storage at +8°C for 6 weeks BVDV detection in leukocytes is not significantly influenced.

Flow cytometry provides a quantitative, objective analysis of leukocyte associated BVDV-antigen. Both low levels of antigen per cell and low percentages of antigen positive cells are detectable. Direct leukocyte analysis with flow cytometry is a rapid (2 hours). easy and reliable method for detection of viremic cattle.

Cover image of the First Volume of the 25th Conference Proceedings: An earl-morning photo of farmland. A small farm is nestled among rolling hills, fields stretching across a curving road that disappears between dark green trees. The barn is a cheery red color with a tin roof. Beneath the photo is the a statement of the conference's theme in white italics: Challenges of the Bovine Industry in the Twenty-First Century. Deep red background.

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Published

1992-08-31

Issue

1992: Volume 1

Section

Endemic Virus Diseases