Bovine myeloid antimicrobial peptide-28 (BMAP-28) mRNA expression by bovine cells and effects of synthetic MAP-28 on bovine respiratory disease pathogens

Authors

  • S. Cornejo Department of Pathobiology and Population Medicine, Mississippi State University, Mississippi State, MS 39762
  • C. Barber Department of Pathobiology and Population Medicine, Mississippi State University, Mississippi State, MS 39762
  • M. Thoresen Department of Pathobiology and Population Medicine, Mississippi State University, Mississippi State, MS 39762
  • D. Vannover Wallace H. Coulter Department of Biomedical Engineering, Georgia Tech and Emory University, Atlanta, GA 30332
  • H. Peck Wallace H. Coulter Department of Biomedical Engineering, Georgia Tech and Emory University, Atlanta, GA 30332
  • P. Santangelo Wallace H. Coulter Department of Biomedical Engineering, Georgia Tech and Emory University, Atlanta, GA 30332
  • A. Woolums Department of Pathobiology and Population Medicine, Mississippi State University, Mississippi State, MS 39762

DOI:

https://doi.org/10.21423/aabppro20228646

Abstract

Mannheimia haemolytica (MH) is the principal bacterial patho­gen associated with bovine respiratory disease (BRD) in beef cattle. Bovine Herpes virus type 1 (BHV-1) can cause BRD by itself or it can predispose cattle to BRD due to MH or other op­portunistic bacteria. Existing antimicrobials do not consis­tently prevent BRD due to MH, and do not have an effect against viruses; bovine antimicrobial peptides (AMP) have immune stimulating and nonspecific antimicrobial effects that could improve BRD control. Messenger RNA (mRNA) treatment could be used to induce AMP expression in cattle, but efficacy must first be confirmed in vitro. Synthetic AMP can be generated to use as standards when characterizing mRNA-expressed AMP. We hypothesized that bovine cells can express synthetic mRNA coding for the AMP BMAP-28 and that synthetic BMAP-28 can inhibit the growth of MH and elicit antiviral effects against BHV-1 virus.

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Published

2023-07-17

Issue

No. 55 (2022): 2022 Annual Conference Proceedings

Section

Research Summaries