Rapid Detection of Bovine Viral Diarrhea Virus using a Conductometric Biosensor

Authors

  • D. L. Grooms Department of Large Animal Clinical Sciences, Michigan State University, East Lansing, MI
  • Z. Muhamammad-Tahir Department of Agriculture Engineering, Michigan State University, East Lansing, MI
  • E. C. Alocilja Department of Agriculture Engineering, Michigan State University, East Lansing, MI

DOI:

https://doi.org/10.21423/aabppro20044930

Keywords:

Bovine viral diarrhea virus, BVDV, carriers, persistently infected, conductometric biosensor, field based applications

Abstract

Bovine viral diarrhea virus (BVDV) is one of the most important viral pathogens of cattle worldwide. A key to controlling BVDV is identifying and eliminating carriers of the virus, better known as cattle persistently infected (PI) with BVDV. PI's shed large amounts of virus and serve as the major source of virus transmission within and between farms. Several laboratory methods are currently available to detect PI's including virus isolation, antigen capture ELISA's, skin immunohistochemistry/fluorescent antibody and PCR. The development of a rapid and cost effective field based system to detect PI's would be beneficial for more practical implementation of BVDV control strategies. In this study, a conductometric biosensor previously developed for use in detecting foodborne pathogens was adapted to detect BVDV in cell culture media and blood. The architecture of this biosensor allows for rapid field based applications.

Cover image of the 37th Conference Proceedings: Cattle in pens that stretch to the horizon. Yellow background.

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Published

2004-09-23

Issue

2004

Section

Research Summaries - Beef

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