Evaluation of Three Cow-side Tests for Detection of Subclinical Ketosis in Fresh Cows
DOI:
https://doi.org/10.21423/aabppro20044937Keywords:
ketosis, cow-side diagnostic tests, acetoacetate, powder test, test strip, BHBAAbstract
Subclinical ketosis in dairy cattle can lead to economic losses through decreased milk production, decreased reproductive performance, increased risk of displaced abomasum and increased risk of clinical ketosis. For early detection of the disease, there is a need for rapid and accurate diagnostic tests.
The objective of the study was to evaluate the performance of three cow-side diagnostic tests for the detection of subclinical ketosis in fresh dairy cows, compared to the gold standard serum β-hydroxybutyrate (BHBA). The cow-side tests were: (1) a commonly used test strip detecting acetoacetate in urine (Ketostix, Bayer Corporation, Elkhart, Indiana, USA), (2) a commonly used powder test used on milk, also detecting acetoacetate (KetoCheck, Great States Animal Health, St. Joseph, Missouri, USA), and (3) a milk test strip detecting BHBA (KetoTest, Sanwa Kagaku Kenkyusho Co. Ltd., Nagoya, Japan, distributed by Elanco Animal Health/Provel, Guelph, Ontario, Canada).