Procedures for Collection and Shipment of Samples for Trichomonas and Campylobacter Culturing from Bulls

Abstract

Equipment and Materials Needed
1. Uterine infusion pipettes. Pipettes with flexible syringe adapters work much better that pipettes with bored syringe adapters.
2. IO- I 2cc syringes.
3. Large diameter plastic soft-drinking straws. Must be large enough to accomodate uterine infusion pipette.
4. 5cc plastic or glass tubes containing . 2cc of USP Lactated Ringer's or 2cc USP Saline Solution. Tubes must be water tight. Red topped 5cc bleeding vacutainer tubes work well. Do not flush samples into bangs bleeding tubes.
5. Insulate box to keep samples from excessive heat or cold during collection and transport to laboratory. Samples for Camypylobacter should be kept at around room temperature.
6. Clark's media in sealed, rubber stopper vials.