Validation of Immulite 2000XPI chemiluminescent immunoassay for cattle serum progesterone measurement

Authors

  • A. A. Megahed Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610
  • K. Jones Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610
  • R. S. Bisinotto Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610
  • K. Pohler Department of Animal Science, Agriculture and Life Sciences, Texas A&M University, College Station, TX 77843
  • J. H. J. Bittar Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610

DOI:

https://doi.org/10.21423/aabppro20228685

Abstract

Monitoring circulating progesterone concentration (P4) is an important component of basic and applied reproduction re­search and clinical settings. Radioimmunoassay (RIA) is con­sidered the gold standard method for measuring P4 in cattle blood, but it generates radioactive waste, has low throughput, and requires licensed facilities. Immulite® 2000XPI (Siemens) is a fully automated immunoassay system marketed for human use to measure concentrations of different analytes including P4. Our objectives were therefore to: 1) validate cattle serum P4 measurement with the Immulite 2000XPI; 2) characterize the analytical performance of the Immulite 2000XPI P4 immunoas­say (IPI) across the reportable concentration range.

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Published

2023-07-17

Issue

No. 55 (2022): 2022 Annual Conference Proceedings

Section

Research Summaries