AMR patterns and RAPD profiles of Streptococcus uberis strains isolated from a clinical bovine mastitis outbreak

Authors

  • Valentina Monistero Department of Veterinary Medicine, University of Milan, Lodi, LO, 26900, Italy
  • P. Cremonesi Institute of Agricultural Biology and Biotechnology, National Research Council, Lodi, LO, 26900, Italy
  • S. Morandi Institute of Sciences of Food Production, National Research Council, Milan, MI, 20133, Italy
  • A. Barberio Istituto Zooprofilattico Sperimentale delle Venezie, Sezione Territoriale di Padova, Legnaro, PD, 35020, Italy
  • C. Locatelli Department of Veterinary Medicine, University of Milan, Lodi, LO, 26900, Italy
  • R. Piccinini Department of Veterinary Medicine, University of Milan, Lodi, LO, 26900, Italy
  • M.F. Addis Department of Veterinary Medicine, University of Milan, Lodi, LO, 26900, Italy
  • P. Moroni Quality Milk Production Services, Animal Health Diagnostic Center, Cornell University, Ithaca, NY, 14850, USA

Keywords:

mastitis, Streptococcus uberis, genotyping

Abstract

Streptococcus uberis is globally recognized as an environmental mastitis pathogen but some strains show a contagious transmission (Wald et al., 2020). Molecular genotyping contributes to understand their modes of dissemination and represents an important tool in epidemiological studies of Strep. uberis mastitis. Random Amplified Polymorphic DNA (RAPD) -PCR is a rapid and inexpensive subtyping technique to detect differences in Strep. uberis (Zadoks et al., 2003). This method was shown to be efficient in identifying clonal strains carried by cows within a farm, whose high prevalence could suggest their role in the intramammary infection spreading (Tomazi et al., 2019). The antimicrobial therapy is still the main strategy for their control and treatment, but the widespread use of drugs has led to an increasing diffusion of Strep. uberis antimicrobial resistance (AMR), resulting in several cow health problems (Pol and Ruegg, 2007).
The antimicrobial susceptibility and the genotypic variability of Strep. uberis strains isolated from a bovine clinical mastitis (CM) outbreak were determined with the aim to investigate the dynamics of Strep. uberis infections at herd level.

Downloads

Published

2021-10-09

Issue

2021 Annual Conference Proceedings

Section

Research Summaries