Evaluation of Two Chute-Side Tests for the Detection of Leukocytes and Bacteria in Semen from Yearling Bulls

Authors

  • M. W. H. Hiew Department of Veterinary Clinical Sciences, Purdue University, West Lafayette, IN 47907
  • L. A. Horstman Department of Veterinary Clinical Sciences, Purdue University, West Lafayette, IN 47907
  • W. L. Singleton Department of Animal Sciences, Purdue University, West Lafayette, IN 47907
  • J. B. Messick Department of Comparative Pathobiology, Purdue University, West Lafayette, IN 47907
  • P. D. Constable Department of Veterinary Clinical Sciences, Purdue University, West Lafayette, IN 47907

DOI:

https://doi.org/10.21423/aabppro20114039

Keywords:

leukocytes, semen, chute-side tests, leukospermia, bacteriospermia, esterases, somatic cells

Abstract

The presence of leukocytes in semen causes bulls to potentially be unsatisfactory breeders. Multistix® 10 SG Reagent Strips (Bayer Healthcare, Elkhart, IN) and the Uriscreen™ catalase test (Jant Pharmacal Corporation, Encino, CA) were used to evaluate their possibility to serve as chute-side tests to detect leukospermia and bacteriospermia in yearling bulls. The reagent strips detect esterases produced by granulocytes, whereas the catalase test reportedly detects the presence of somatic cells (> 10 cells per high power field) or bacteria (>5 x 104 CFU/ml).

Cover image of the 44th Conference Proceedings: photo of the skyline of St. Louis, Missouri, site of the 2011 AABP Conference. Yellow background.

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Published

2011-09-22

Issue

2011

Section

Research Summaries 3

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