Enterite Hemorragique D'Hiver en France

Confirmation de Son Association Avec Un Coronavirus Par Microscopie Electronique Et Par Culture Sur Cellules Hrt 18

Authors

  • R. Carnero Unité de Virologie, LPB/CNEVA
  • C. Costes Unité de Virologie, LPB/CNEVA
  • F. Schelcher Département de Physiopathologie Animale, Ecole Nationale Vétérinaire, 23 chemin des Capelles, 31076 Toulouse Cedex
  • J. -F. Valarcher Département de Physiopathologie Animale, Ecole Nationale Vétérinaire, 23 chemin des Capelles, 31076 Toulouse Cedex
  • M. Moulignie Département de Physiopathologie Animale, Ecole Nationale Vétérinaire, 23 chemin des Capelles, 31076 Toulouse Cedex
  • J. Espinasse Département de Physiopathologie Animale, Ecole Nationale Vétérinaire, 23 chemin des Capelles, 31076 Toulouse Cedex

DOI:

https://doi.org/10.21423/aabppro19926605

Keywords:

winter dysentery, coronavirus, immunofluorescence, calf diarrhea coronavirus

Abstract

 During the 1990-1991 winter, 59 samples of faeces were made in cattle suffering from winter dysentery. These animals were coming from 8 different farms located in 6 different french departments. Each sample has been studied carefully under the electron microscope and put for culture on HRT 18 cells. 32 samples (54%) have shown coronavirus-like viral particles under the microscope. Each group of animals coming from one farm having at least 2 samples found(+) . 6 isolates have been kept from the cells culture and studies after the 9th passage. In these 6 isolates coronavirus were identified with the the help of the electron microscope, their cytopathogenic effect and its seroneutralization, and an direct immunofluorescence method using an anticoronavirus serum prepared on rabbits with a calf diarrhea coronavirus vaccinal strain. These results are in accordance with a participation of this type of virus in the winter dysentery syndrome.

Full article in French, English and Spanish summaries on PDF

Cover image of the Third Volume of the 25th Conference Proceedings: An earl-morning photo of farmland. A small farm is nestled among rolling hills, fields stretching across a curving road that disappears between dark green trees. The barn is a cheery red color with a tin roof. Beneath the photo is the a statement of the conference's theme in white italics: Challenges of the Bovine Industry in the Twenty-First Century. Deep red background.

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Published

1992-08-31

Issue

1992: Volume 3

Section

Virus Diseases

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