Mastitis microbiology simplified
DOI:
https://doi.org/10.21423/bovine-vol0no21p77-78Keywords:
coliform bacteria, Cows, differentiation, Mastitis, microorganismsAbstract
A system for determining whether mastitis is due to contagious or environmental pathogens is described. The sampling format for herds with bulk milk somatic cell counts (SCC) >500 000/ml or Wisconsin mastitis test (WMT) score >15 is to test all cows with high SCC or WMT and a random sample of cows representative of the total herd with respect to age and lactation stage; for herds with clinical mastitis cases but bulk milk SCC <300 000 and WMT <10, samples are taken from cows with high SCC or WMT and from those with clinical mastitis. Bacterial culturing of the samples on sheep blood agar, thallium/crystal violet/ferric citrate agar and McConkey's agar allows determination of whether Streptococcus spp., Staphylococcus spp. or coliforms are present; catalase, coagulase and cAMP tests provide further classification of the mastitis pathogens present.