Evaluation and validation of a paralumbar fossa transabdominal rumen fluid sampling technique


  • Jerry R. Roberson College of Veterinary Medicine, Lincoln Memorial University, Harrogate, TN 37752
  • Hilari E. French Department of Clinical Sciences, Ross University School of Veterinary Medicine, St. Kitts, West Indies
  • James Q. Robinson LaGrange Veterinary Clinic, 1005 North Detroit, LaGrange, IN 46761




cattle, rumen fluid, rumen evaluation, rumen sampling technique


A simple method to obtain rumen fluid via the left paralumbar fossa was evaluated on 58 adult cattle. Using a 16-gauge 1.5 inch (3.8 cm) needle, rumen fluid was successfully collected and evaluated from 45 of 58 head (78%). The primary reason for failure was body wall thickness exceeding the 1.5 inch (3.8 cm) needle (P = 0.0002). Average rumen pH of the 45 cattle samples was 7.9; the test cattle were primarily fed guinea grass with occasional brewer's grain supplement. An average 3 protozoa per field under 40 x magnification were seen. No external hematomas or other swellings were seen during the 3-week follow-up observations, and no complications were noted. Eleven of the 58 cattle (19%) had increased body wall thickness, based on ultrasonographic follow-up. The average length of time required to obtain a rumen sample, measured by introduction of the needle to the withdrawal of the needle, was 3 seconds (range< 1 to 8 sec). We conclude that the left paralumbar fossa trans-abdominal rumen fluid sampling technique is a safe and efficient method to obtain rumen fluid in cattle with a moderately thin (~ 30 mm) body wall.




How to Cite

Roberson, J. R., French, H. E., & Robinson, J. Q. (2017). Evaluation and validation of a paralumbar fossa transabdominal rumen fluid sampling technique. The Bovine Practitioner, 51(1), 73–76. https://doi.org/10.21423/bovine-vol51no1p73-76