A Preliminary evaluation of the effect of vaccination with modified live bovine viral diarrhea virus (BVDV) on detection of BVDV antigen in skin biopsies using immunohistochemical methods

Authors

  • William R. DuBois Oklahoma State University, Department of Veterinary Clinical Sciences
  • V. L. Cooper Oklahoma State University, Oklahoma Animal Disease Diagnostic Laboratory
  • Jeanenne C. Duffy Oklahoma State University, Oklahoma Animal Disease Diagnostic Laboratory
  • Darrin D. Dean Oklahoma State University, Oklahoma Animal Disease Diagnostic Laboratory
  • Roy L. Ball Oklahoma State University, Willard Sparks Beef Research Center
  • Billie D. Starr Oklahoma State University, Willard Sparks Beef Research Center

DOI:

https://doi.org/10.21423/bovine-vol34no2p98-100

Keywords:

biopsy, calves, diagnosis, diagnostic techniques, immunohistochemistry, live vaccines, mucosal disease, respiratory diseases, vaccination, vaccines

Abstract

Immunohistochemical (IHC) testing of skin biopsies (ear notch samples) is a method to identify cattle persistently infected (PI) with bovine diarrhoea virus (BDV). Skin biopsies were taken from 90 calves upon arrival (day 0) at a feedlot, and on days 14, 28 and 42 following arrival. Calves were vaccinated with modified live virus (MLV) BDV vaccine on days 1 and 14 following arrival. Additional samples were obtained from 50 of the calves between days 1 and 10 when they were treated for bovine respiratory disease. Immunohistochemical testing for BDV antigen was performed on all samples. One calf was positive for BDV antigen by IHC techniques on 5 skin biopsies. This calf was also positive by virus isolation (VI) on the buffy coat of a blood sample obtained on day 42 following arrival. All samples from the remaining calves were negative when tested with the IHC method. Other tests, VI and polymerase chain reaction, used to identify PI cattle may give positive results in cattle recently vaccinated with MLV BDV vaccine. This study suggests that IHC testing of skin biopsies may be advantageous over other tests to identify PI cattle recently vaccinated with MLV BVDV.

Cover image of Volume 34, no.2 of the Bovine Practitioner: three photographs placed on top of an image of Mt. Rushmore at dawn. From top clockwise the photographs are of a herd of bison grazing on a field, a view of the badlands national park at sunrise, and the statue of Crazy Horse Monument with the view of the unfinished monument in the background

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Published

2000-05-01

How to Cite

DuBois, W. R., Cooper, V. L., Duffy, J. C., Dean, D. D., Ball, R. L., & Starr, B. D. (2000). A Preliminary evaluation of the effect of vaccination with modified live bovine viral diarrhea virus (BVDV) on detection of BVDV antigen in skin biopsies using immunohistochemical methods. The Bovine Practitioner, 34(2), 98–100. https://doi.org/10.21423/bovine-vol34no2p98-100

Issue

Vol. 34, No. 2 (2000 May)

Section

Articles