Rapid diagnosis of rotavirus by an enzyme-linked immunosorbent assay (ELISA) in an outbreak of neonatal diarrhea in dairy calves

Authors

  • S. J. McGuire Oklahoma Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma 74078
  • A. E. Castro Oklahoma Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma 74078

DOI:

https://doi.org/10.21423/bovine-vol1982no17p66-71

Keywords:

calves, cattle diseases, Colostrum, Diagnostic techniques, diarrhoea, ELISA, Newborn animals, viral diseases

Abstract

An outbreak of diarrhoea affected 13 of 20 calves in a herd of 260 cows and heifers. Faeces from 10 of the 13 calves were positive or doubtful for rotavirus group-specific (gs) antigens by ELISA. Faeces from 4 cows, including the dam of one affected calf, were all negative. Cell cultures of faeces from 3 affected calves were negative for rotavirus by immunofluorescence. Supernatant fluid from a culture of one calf was positive for gs antigens by ELISA. All 13 affected calves recovered after receiving antibiotics and colostrum. One calf, vaccinated against rotavirus at birth, had slight diarrhoea but recovered. ELISA is recommended for rapid diagnosis of rotavirus in calf diarrhoea.

Cover image of the 17th Volume of the Bovine Practitioner: a photo of the Nashville, Tennessee Skyline with inset Photos of Opryland and Opryhouse.

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Published

1982-11-01

How to Cite

McGuire, S. J., & Castro, A. E. (1982). Rapid diagnosis of rotavirus by an enzyme-linked immunosorbent assay (ELISA) in an outbreak of neonatal diarrhea in dairy calves. The Bovine Practitioner, 1982(17), 66–71. https://doi.org/10.21423/bovine-vol1982no17p66-71

Issue

No. 17 (1982 November)

Section

Articles